This is the original main line of the Virginia and Tennessee
| Station | Miles From Radford | Telegraph Signal | Notes |
|---|---|---|---|
| East Radford | 0 | CN | |
| Radford | 1.2 | R | Changed to JC in TT #12 |
| W. End Double Track | 1.5 | JC | Removed in TT #12 |
| New River | 2.3 | NR | |
| Melborn | 5.4 | -- | |
| Dublin | 8.0 | BN | Station is still standing |
| Wurno | 11.6 | -- | |
| North Carolina Junction | 13.9 | CR | North Carolina Branch |
| Dora | 13.9 | CR | Name changed in TT #16 |
| Pulaski | 15.3 | SK | Changed to DI in TT #4, to JN in TT #6 |
| Station has been restored as a visitor's center | |||
| Clark's Tank | 22.1 | -- | Changed to Clark in TT #16 |
| Summit | 22.9 | -- | |
| Max Meadows | 28.0 | MX | |
| Kent | 32.8 | -- | |
| Wytheville | 36.0 | W | Station is still standing |
| Grubb | 41.1 | -- | |
| Crockett | 43.8 | -- | |
| Rural Retreat | 49.0 | AY | |
| Station is still standing; same construction as AM&O stations in Christiansburg and Concord. O. Winston Link took several photographs here and made recordings of steam engines climbing the grade to Rural Retreat. | |||
| Grosclose | 53.0 | -- | Changed to GQ in TT #16 |
| Atkins | 57.8 | KN | |
| Holston | 63.1 | -- | |
| Marion | 63.6 | ND | Station is still standing. |
| Seven Mile Ford | 70.7 | FD | |
| Chilhowie | 74.1 | CO | |
| Glade Spring | 79.7 | GS | Saltville Branch |
| Emory | 83.2 | -- | Station is used by Emory and Henry College. |
| Meadow View | 85.2 | CD | |
| Abingdon | 93.0 | Q | Freight station is a craft shop. |
| Wyndale | 98.8 | Z | |
| Wallace | 102.1 | WC | |
| Bristol | 107.7 | BD | Station has been restored and is a downtown shopping mall. |
| Station | Miles From Glade Spring | Telegraph Signal | Notes |
|---|---|---|---|
| Glade Spring | GS | ||
| Saltville | 9.4 | SV |