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2 Invited Editorials          

  • 1. Zhang Y-HP*, 2008. A sweet solution. Public Service Review: European Union. Science & Technology (in press, PDF).
  • 2. Zhang Y-HP*, 2008. Driving tomorrow by sugars. Public Service Review: European Union. Science & Technology (in press, PDF).

31 Peer-Reviewed Journal Publications 

    Year 2008

  • 31.Moxley G, Zhu Z, Zhang Y-HP*. 2008. Efficient sugar release by the cellulose solvent-based lignocellulose fractionation technology and enzymatic cellulose hydrolysis. Journal of Agriculture and Food Chemistry (PDF).

  • 30. Hong J, Ye X, Wang Y, Zhang Y-HP*. 2008. Bioseparation of recombinant cellulose binding module-protein by affinity adsorption on an ultra-high-capacity cellulosic adsorbent. Analytica Chimica Acta 621:193-199 (PDF) .

  • 29. Hong J, Wang Y, Ye X, Zhang Y-HP*. 2008. Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage. Journal of Chromatography A. 1194(2): 150-154 (PDF).

  • 28. Zhang Y.-H.P.  2008. Reviving the carbohydrate economy via multi-product biorefineries. Journal of Industrial Microbiology and Biotechnology: 35 (5): 367-375 (PDF).

  Year 2007

  • 27. Hong J., Ye X-H., Zhang Y.-H.P.  2007. Quantitative determination of cellulose accessibility to cellulase based on adsorption of a non-hydrolytic fusion protein containing CBM and GFP with its applications. Langmuir 23 (25): 12535-12540 (PDF).

  • 26. Moxley G, Zhang Y.-H.P. 2007. More accurate determination of acid-labile carbohydrate composition in lignocellulose by modified quantitative saccharification. Energy and Fuels 21: 3684-3688 (PDF).

  • 25. Zhang Y.-H.P., Mielenz J.R., Evans B.R., Hopkins R.C., Adams M.W.W. 2007. High-yield hydrogen production from starch and water by synthetic enzymatic pathway. PLoS One 2(5): e456 (PDF). Note: This is a milestone paper for linking the future carbohydrate and hydrogen economy. Also, we proposed a new concept technology -- cell free synthetic enzymatic pathway for complicated biotransformation.
  • 24. Zhang Y.-H.P., Ding S.-Y., Mielenz J.R., Cui J.-.B., Elander R.T., Laser M, Himmel M.E., McMillan, J.D., Lynd, L.R. 2007. Fractionating recalcitrant lignocellulose at modest reaction conditions. Biotechnology and Bioengineering 97(2): 214-223 (PDF). Note: This is the most downloaded paper in year 2007 and highlighted as a special Free Download paper selected by Jone Wiley & Son and ChemWeb.
  • 23. Zhang Y.-H.P., Schell D.R. McMillan J.D. 2007. Methodological analysis for determination of enzymatic digestibility of cellulosic materials. Biotechnology and Bioengineering 96(1): 188-194 (PDF).

    Year 2006

  • 22. Lu Y.P., Zhang Y.-H.P., Lynd L.R. 2006. Evidence for enzyme-microbe synergy in cellulose utilization by Clostridium thermocellum. Proceedings of the National Academy of Sciences of USA 103(44): 16165-16169 (PDF) .

  • 21. Zhang Y.-H.P., Himmel E. M., Mielenz J.R. 2006. Outlook for cellulase improvement: Screening and selection strategies. Biotechnology Advances, 24(5): 452-481 (PDF).

  • 20. Zhang Y.-H.P., Lynd L.R. 2006. A functionally-based model for hydrolysis of solid cellulose by fungal cellulase. Biotechnology and Bioengineering, 94(5): 888-898 (PDF).

  • 19. Zhang Y.-H.P., Cui X.B., Lynd L.R., and Huang L. 2006. A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: Evidences from supramolecular structures and enzymatic hydrolysis. Biomacromolecules 7(2): 644-648 (PDF).

  • 18. Zhang Y.-H.P., and Lynd L.R. 2006. Biosynthesis of radio-labeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugars.  Applied Microbiology and Biotechnology 70(1):123-129 (PDF).  

    Year 2005

  • 17. Zhang Y.-H.P., and Lynd L.R. 2005. Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation.  Proceedings of the National Academy of Sciences of USA 102:7321-7325 (PDF).

  • 16. Zhang Y.-H.P., and Lynd L.R. 2005. Determination of the number average degree of polymerization of cellodextrins and cellulose with application to enzymatic cellulose hydrolysis  Biomacromolecules 6: 1510-1515 (PDF).

  • 15. Zhang Y.-H.P., and Lynd L.R. 2005. Regulation of cellulase biosynthesis in batch and continuous cultures of Clostridium thermocellum.  Journal of Bacteriology 187:99-106 (PDF).

    Year 2004

  • 14.  Zhang Y.-H.P., and Lynd L.R. 2004. Toward an aggregated understanding of enzymatic hydrolysis of cellulose: non-complexed cellulase systems.  Biotechnology and Bioengineering 88:797-824 (PDF). Note: One of the most downloaded paper (top 25).

  • 13. Zhang Y.-H.P., and Lynd L.R. 2004. Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum.   Applied and Environmental Microbiology 70:1563-1569 (PDF).

    Year 2003

  • 12. Zhang Y.-H.P., and Lynd L.R. 2003. Cellodextrin production from mixed-acid hydrolysis and chromatographic separation.   Analytical Biochemistry 332:225-232 (PDF).

  • 11. Zhang Y.-H.P., and Lynd L.R. 2003. Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an ELISA-based method with application to Clostridium thermocellum batch Cultures.   Analytical Chemistry 75:219-227 (PDF).

    Year 2002

  • 10. Lynd L.R., and Zhang Y.H. 2002. Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: Analytical framework and methodological approach.   Biotechnology and Bioengineering 77:467-475 (PDF).

    Year 2001

  • 9. Ozkan M., Desai S.G., Zhang Y.-H., Stevenson D.M., Beane J., Guerinot M.L., and Lynd L.R. 2001. Characterization of thirteen newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria.   Journal of Industrial Microbiology Biotechnology 27:275-280 (PDF).

   Before Year 2000

  • 8. Zhang Y.-H., and Zhong J.-J. 1997. Hyper-production of ginseng saponin and polysaccharide production by high-density cultivation of Panax notoginseng cells.   Enzyme and Microbial Technology 21:58-63 (PDF).

  • 7. Zhang Y.-H., Wang H.-Q., Liu S., Yu J.-T., and Zhong J.-J. 1997. Regulation of apparent viscosity and oxygen transfer coefficient by osmotic pressure in cell suspension of Panax notoginseng.   Biotechnology Letters 19: 943-945 (PDF).

  • 6. Zhong J.-J., Meng X.-D., Zhang Y.-H., and Song L. 1997. Effective release of ginseng saponin from suspension cells of Panax notoginseng.   Biotechnology Techniques 11:241-243. (PDF)

  • 5. Liu S., Zhong J.-J., Zhang Y.-H., and Meng X.-D. 1997. Effective release of ginseng saponin in cell suspension cultures of Panax notoginseng permeated with dimethyl sulfoxide.   Journal of ECUST 23:684-687 (Chinese).

  • 4. Zhang, Y.-H., Zhong, J.-J., and Yu, J.-T. 1997. High-density cultivation of Panax notoginseng cells for production of ginseng saponin and polysaccharide.   Journal of ECUST 23:310-314 (Chinese).

  • 3. Zhang, Y.-H., Zhong, J.-J., and Yu, J.-T. 1996. Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: medium sucrose manipulation.   Journal of Biotechnology 51: 49-56 (PDF).

  • 2. Zhang Y.-H., Zhong J.-J., and Yu J.-T. 1996. Effect of nitrogen source on cell growth and ginseng saponin and polysaccharide in suspension cell cultures of Panax notoginseng.   Biotechnology Progress 12: 567-571 (PDF).

  • 1. Zhang Y.-H., Zhong J.-J., and Yu J.-T. 1995. Effects of osmotic pressure on cell growth and ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng.   Biotechnology Letters 17: 1347-1350.

    

Six Book Chapters     

  • Zhang Y.-H.P., Jiong Hong, Yinhao Ye.  2007. Cellulase Assays in Methods in Molecular Biology: Biofuels (ed. by Mielenz, J.R.) The Humana Press Inc., Totowa, NJ (in production).

  • Zhang Y.-H.P., Lynd LR. 2007. New generation biomass conversion: Consolidated bioprocessing in Biomass Recalcitrance:  Deconstructing the Plant Cell Wall for Bioenergy (ed. by Himmel, M.E.) Blackwell Publishing. ISBN: 9781405163606. pp 480-493.

  • Lynd L.R.; Weimer P.J.; Wolfaardt G.; Zhang Y.-H.P. 2006. Cellulose hydrolysis by Clostridium thermocellum: A microbial perspective. In Cellulosome (ed. by Kataeva, I.A.). Nova Science Publishers, Inc. Hauppauge, NY, U.S.A. ISBN: 1594549508. pp 95-117.         

Eleven Patent Disclosures

  • 5. Zhang Y.-H.P. and Mielenz J.R. 2006. Biohydrogen production from polysaccharides by artificial enzymatic pathway (filed jointly by Virginia Tech and Oak Ridge National Laboratory).
  • 4. Lynd LR, Lu Y, Zhang Y.-H.P. 2006. Selection of microorganisms with growth dependent upon extracytoplasmic enzymes. Patent Application Serial No 60/796,635 by Dartmouth College on May 1, 2006.
  • 3. Zhang Y.-H.P. 2006. A novel cellulose-solvent-based lignocellulose pretreatment (filed as the global patent with the disclosure 05.087 by Virginia Tech).
  • 2. Zhang Y.-H.P., and Lynd L.R. 2005.  Method and apparatus for saccharide precipitation from pretreated lignocellulose materials. US Patent Provisional Filing. 
  • 1. Zhang Y.-H.P., and Lynd L.R. 2004. Cellodextrin production from mixed-acid hydrolysis and chromatographic separation. US Patent Provisional No. 20040217063.  

    Selected Presentation Slides

  • Zhang Y.-H.P. Effectively Fractionating Lignocellulose and Releasing Sugars by Enzymatic Hydrolysis. The 29th Symposium on Biotechnology for Fuels and Chemicals. Nashville, Tennessee, May 2, 2007 (Oral Presentation 5B-05).

  • Zhang Y.-H.P. Developing zero-pollution cars powered by sugars. Virginia Tech Dean’s Environment Forum. Blacksburg, VA., Feb. 26, 2007. First Place Faculty Award in the Environmental Technologies. (Science Fiction or Reality -- Future Vehicles Powered by Sugar).

 

© 2005 Y-H Percival Zhang. All rights reserved.