Note: All publication reprints are available upon request.
A. Peer-reviewed Journal Publications and Book Chapters
121. Zhang X-Z, You C, Zhang Y-HP*. 2013. Simple and Fast Transformation of Bacillus subtilis. In Methods of Molecular Biology, In press (Invited book chapter).
120. Zhu ZG, Tam TK, Sun FF, You C, Zhang Y-HP*. 2014. A high-energy-density sugar biobattery based on a synthetic enzymatic pathway. Nature Communications. 5: 3026 (PDF). (This paper has an altmetric score of 300+). In this study, we achieve the highest energy density battery in the world, higher than any primary, secondary batteries and fuel cells. In addition, this battery features biodegrability, 100% safety, and fast rechargeability.
Time (magazine) -- A Sugar-Powered Smartphone? Sweet!
BBC -- Scientists have created a sugar-powered battery for our smartphones
US News and World Report -- Spoonfuls of Sugar Could Soon Power Devices
UPI -- Researchers harness sugar's 'perfect energy storage' capacity to build battery
Business Insider -- A Battery That Runs On Sugar Could Soon Be Powering Your Electronics
ExtremeTech -- Sugar-powered biobattery has 10 times the energy storage of lithium: Your smartphone might soon run on enzymes
TheGuardian -- Sugar battery offers hope of green-powered gadgets within three years
RedOrbit -- Researchers develop energy-dense sugar battery
The Engineer – Fuel cell receives energy boost from sugar.
119. You C, Zhang Y-HP*. 2014. Simple cloning and DNA assembly in E. coli by prolonged overlap extension PCR. Methods of Molecular Biology 1116; DOI: 10.1007/978-1-62703-764-8_13 (PDF).
118. Gao SH, You C, Renneckar S, Bao J, Zhang Y-HP*. 2014. New insights into enzymatic hydrolysis of heterogeneous cellulose by using CBM3-containing GFP and CBM17-containing CFP. Biotechnology for Biofuels accepted.
117. You C, Zhang Y-HP*. 2014. Annexation of a high-activity rate-limiting enzyme in a synthetic three-enzyme complex greatly decreases the degree of substrate channeling. ACS Synthetic Biology Epub, DOI: 10.1021/sb4000993 (PDF).
116. Ahmad S, Ma H, Akhtar MW, Zhang Y-HP*, Zhang X-Z*. 2014. Directed evolution of Clostridium phytofermentans glycoside hydrolase family 9 endoglucanase for enhanced specific activity on solid cellulosic substrate. Bioenergy Research, Epub (PDF).
115. Zhang XZ, Zhang Y-HP*. 2012. Cellulases: characteristics, sources, production and applications. Bioprocessing Technologies in Integrated Biorefinery for Production of Biofuels, Biochemicals, and Biopolymers from Biomass (ed. by Yang ST, El Enshasy HA, Thongchul N, Lo YM). Wiley Pp131-146. ISBN-13: 9780470541951.
114. Sathitsuksanoh N*, Xu B, Zhao BY, Zhang Y-HP. 2013. Overcoming biomass recalcitrance by combining genetically modified switchgrass and cellulose solvent-based lignocellulose pretreatment. PLoS One 8(9):e73523 (PDF).
113. Myung S, You C, Zhang Y-HP*. 2013. Recyclable cellulose-containing magnetic nanoparticles: immobilization of cellulose-binding module-tagged proteins and synthetic metabolon featuring substrate channeling. Journal of Materials Chemistry B. 1:4419-4427 (PDF).
112. Rollin JR, Tam TK, Zhang Y-HP*. 2013. A biotechnology paradigm: cell-free biosystems for biomanufacturing. Green Chemistry. 15:1708-1719 (PDF) (invited).
111. Zhang Y-HP*. 2013. Next-generation biorefineries will solve the food, biofuels and environmental trilemma in the energy-food-water nexus. Energy Science and Engineering: 1: 25-41 (PDF). (Invited).
110. You C, Chen HG, Myung S, Sathisuksanoh N, Ma H, Zhang XZ, Li JY, Zhang Y-HP*. 2013. Enzymatic transformation of non-food biomass to starch. Proceedings of the National Academy of Sciences of the USA. 110: 7182-7189 (PDF). Highlighted by Science magazine.
Science Magazine -- Could Wood Feed the World?
Popular Science – We could eat trees: Scientists turn inedible plant cellulose into starchy snack
Scientific American (Chinese) -- New biotechnological breakthrough converts biomass to edible food
Voice of America – Chemical process creates food source from plant waste
109. Martin del Campo JS, Rollin JR, Myung S, You C, Chandrayan S, Patiño R, Adams MWW, Zhang Y-HP*. 2013. Dihydrogen production from xylose and water mediated by synthetic cascade enzymes. Angewandte Chemie International Edition 52:4587-4590 (PDF) (Editor’s choice paper). Abstract: Let enzymes work! Dihydrogen was produced from xylose and water in a single reactor containing 13 enzymes. By using a novel polyphosphate xylulokinase (XK), xylose was converted to H2 and CO2 with approaching 100% of the theoretical yield. These findings suggest that cell-free biosystems could produce H2 from biomass xylose at low cost.
Forbes/CBS NEWS/Sky TV -- Could Hydrogen Breakthrough Revive The Fuel-Cell Car?
Phyorg -- Breakthrough in hydrogen fuel production could revolutionize alternative energy market
Biofuel Journal -- Sugar to Hydrogen
UPI -- Scientists make hydrogen fuel from plants
The Verge – Hydrogen fuel breakthrough lets researchers extract gas from any plant
Raw Story -- Biofuel breakthrough turns virtually any plant into hydrogen
108. Martin del Campo JS, You C, Kim J-E, Patiño R, Zhang Y-HP*. 2013. Discovery and characterization of a novel ATP/polyphosphate xylulokinase from a hyperthermophilic bacterium Thermotoga maritima. Journal of Industrial Microbiology and Biotechnology 40:661-669 (PDF).
107. Zhu ZG, Tam TK, Zhang Y-HP*. 2013. Cell-free biosystems for biomanufacturing in the production of electricity and bioenergy. Advances in Biochemical Engineering/Biotechnology DOI: 10.1007/10_2013_201 (Invited).
106. Jandt U, You C, Zhang Y-HP, Zeng A-P*. 2013. Compartmentation and metabolic channeling: practical and modeling aspects for multienzymatic biosynthesis. Advances in Biochemical Engineering/Biotechnology In press (Invited).
105. Myung S, Zhang Y-HP*. 2013. Non-complexed four cascade enzyme mixture: simple purification and synergetic co-stabilization. PLoS ONE 8, e61500 (PDF).
104. Zhang Y-HP*, Xu J-H, Zhong J-J. 2013. A new high-energy density hydrogen carrier - carbohydrate - might be better than methanol. International Journal of Energy Research. 37:769-779 (PDF).
103. You C, Zhang Y-HP*. 2013. Cell-free biosystems for biomanufacturing. Advances in Biochemical Engineering/Biotechnology 131:89-119 (PDF) (Invited).
102. You C, Zhang Y-HP*. 2013. Self-assembly of synthetic metabolons through synthetic scaffoldins: single-step purification, co-immobilization, and substrate channeling. ACS Synthetic Biology 2:102-110 (PDF) (cover page).
101. Sathitsuksanoh N, George A, Zhang Y-HP*. 2013. New lignocellulose pretreatments by using cellulose solvents: a review. Journal of Chemical Technology and Biotechnology 88: 169-180 (PDF) (Invited).
100. You C, Myung S, Zhang Y-HP*. 2012. Facilitated substrate channeling in a self-assembled trifunctional enzyme complex. Angewandte Chemie International Edition.51: 8787-8790 (PDF). Abstract: Dockerin-containing triosephosphate isomerase, aldolase, and fructose 1,6-bisphosphatase were self-assembled into a static trifunctional enzyme complex through a mini-scaffoldin containing three different cohesins. The synthetic enzyme complex exhibited reaction rate enhancement compared to non-complexed three enzyme mixture at the same enzyme concentration by a factor from 10.3 to 21.1 when substrate concentration decreased from 5.0 to 0.5 mM.
99. You C, Zhang Y-HP*. 2012. Easy preparation of a large-size random gene mutagenesis library in Escherichia coli. Analytical Biochemistry 428:7-12 (PDF).
98. Sathisuksanoh N, Zhu ZG, Zhang Y-HP*. 2012. Cellulose solvent-based pretreatment for corn stover and Avicel: concentrated phosphoric acid versus ionic liquid [BMIM]Cl. Cellulose 19: 1161-1172 (PDF).
97. Zhang Y-HP*. 2012. Sustainability of the Sugar and Sugar–Ethanol Industries. ChemSusChem. 5: 1638 (PDF) (Invited Book Review).
96. Sathisuksanoh N, Zhu ZG, Zhang Y-HP*. 2012. Cellulose solvent- and organic solvent-based lignocellulose fractionation enabled efficient sugar release from a variety of lignocellulosic feedstocks. Bioresource Technology 117:228-233 (PDF).
95. Zhang Y-HP*, Chun Y, Chen HG, Feng RL. 2012. Surpassing photosynthesis: high-efficiency and scalable CO2 utilization through artificial photosynthesis. ACS Symposium Series 1097: 275-292 (Recent Advances in Post-Combustion CO2 Capture Chemistry), Oxford University Press, UK. (PDF).
94. Zhu ZG, Sun FF, Zhang XZ, Zhang Y-HP*. 2012. Deep oxidation of glucose in enzymatic fuel cells through a non-natural synthetic enzymatic pathway containing a cascade of two thermostable dehydrogenases. Biosensors and Bioelectronics 36: 110-115 (PDF).
93. Ye X, Zhang CM, Zhang Y-HP*. 2012. Engineering a large protein by combined rational and random approaches: Stabilizing the Clostridium thermocellum cellobiose phosphorylase. Molecular BioSystems, 8: 1815-1823 (PDF).
92. Zhang Y-HP*, Xu J-H, Zhong JJ. 2012. A new high-energy density hydrogen carrier - carbohydrate - might be better than methanol. International Journal of Energy Research. Epub, (PDF).
91. You C, Zhang X-Z, Zhang Y-HP*. 2012. Mini-scaffoldin enhanced mini-cellulosome hydrolysis performance on low-accessibility cellulose (Avicel) more than on high-accessibility amorphous cellulose. Biochemical Engineering Journal 63:57-65 (PDF).
90. Zhang Y-HP*, Huang WD. 2012. Constructing the electricity-carbohydrate-hydrogen cycle for sustainability revolution. Trends in Biotechnology 30: 301-306. (PDF) (Opinion). Abstract: To enhance energy utilization efficiency, we wish to suggest the electricity-carbohydrate-hydrogen (ECHo) cycle bridging among primary energies and secondary energies. Carbohydrates are sources of food, feed, liquid biofuels, and renewable materials and would be a high-density hydrogen carrier and electricity storage compound (e.g., more than 3,000 Wh/kg). One element of this ECHo cycle can be converted to another reversibly and efficiently depending on resource availability, needs, and costs. This cycle would not only supplement current and future primary energy utilization systems for facilitating electricity and hydrogen storage and enhancing secondary energy conversion efficiencies, but also address such sustainability challenges as transportation fuel production, CO2 utilization, fresh water conservation, and maintenance of a small closed ecosystem in emergency situations. Key implication : Carbohydrate could be the best solar fuel, the best long-term electricity storage compound, and sources for food, feed and renewable materials.
89. Sun FF, Zhang XZ, Zhang Y-HP*. 2012. Thermophilic Thermotoga maritima ribolse-5-phosphate isomerase RpiB: Optimized heat treatment purification and basic characterization. Protein Expression and Purification 82:302-307 (PDF).
88. You C, Zhang X-Z, Zhang Y-HP*. 2012. Simple Cloning via direct transformation of PCR product (DNA multimer) to Escherichia coli and Bacillus subtilis. Applied and Environmental Microbiology 78(5):1593-1595 (PDF). This general cloning technology can subclone up to three DNA fragments into any location of a plasmid without the need of restriction enzymes and ligases.
87. You C, Zhang X-Z , Sathitsuksanoh N , Lynd LR, Zhang Y-HP*. 2012. Enhanced microbial cellulose utilization of recalcitrant cellulose by an ex vivo cellulosome-microbe complex. Applied and Environmental Microbiology 78(5):1437-1444 (PDF).
86. Huang SY, Zhang Y-HP, Zhong JJ*. 2012. A thermostable recombinant transaldolase with high activity over a broad pH range. Applied Microbiology and Biotechnology 93(6):2403-2410 (PDF).
85. Wang QQ, He ZB, Zhu ZG, Zhang Y-HP, Ni YH, Luo XL, Zhu JY*. 2012. Evaluation of cellulose accessibilities of lignocelluloses by solute exclusion and protein adsorption techniques. Biotechnology and Bioengineering 109: 381-389 (PDF).
84. Liao HH, Myung S, Zhang Y-HP*. 2012. One-step purification and immobilization of thermophilic polyphosphate glucokinase from Thermobifida fusca YX: glucose-6-phosphate generation without ATP. Applied Microbiology and Biotechnology 93:1109-1117 (PDF).
83. Zhang Y-HP*. 2011. Chapter 8: Hydrogen production from carbohydrates: a mini-review. ACS Symposium Series 1067:203-216 (invited book chapter). (Sustainable Production of Fuels, Chemicals, and Fibers from Forest Biomass), Oxford University Press, UK. ISBN13: 9780841226432. DOI: 10.1021/bk-2011-1067.ch008.
82. Kuhad RC*, Gupta R, Khasa Y, Singh A, Zhang Y-HP. 2011. Bioethanol production from pentose sugars: Current status and future prospects. Renewable and Sustainable Energy Reviews 15:4950-4962 (PDF).
81. Xu B, Escamilla-Trevino L, Noppadon S, Shen Z, Zhang Y-HP, Dixon R, Zhao B*. 2011. RNA interference silencing of 4-coumarate: Coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production. New Phytologist 192: 611-625 (PDF).
80. Zhang Y-HP*. 2011. What is vital (and not vital) to advance economically-competitive biofuels production. Process Biochemistry 46: 2091-2110 (Invited opinion review, PDF). This opinion paper clearly criticize some wrong directions of advanced biofuels and points out vital directions.
79. Zhang Y-HP*, Myung S, You C, Zhu ZG, Rollin J. 2011. Toward low-cost biomanufacturing through cell-free synthetic biology: bottom-up design. Journal of Materials Chemistry 21: 18877-18886 (Invited feature article, PDF).
78. Liao H-H, Zhang X-Z, Rollin J, Zhang Y-HP*. 2011. A minimal set of bacterial cellulases for consolidated bioprocessing of lignocelluloses. Biotechnology Journal 6: 1409-1418 (PDF).
77. Zhang Y-HP*. 2011. Substrate channeling and enzyme complexes for biotechnological applications. Biotechnology Advances 29: 715-725 (PDF).
76. Ye X, Zhu ZG, Zhang CM, Zhang Y-HP*. 2011. Family 9 carbohydrate-binding module improves the catalytic potential of Clostridium thermocellum cellodextrin phosphorylase on cellulosic materials. Applied Microbiology and Biotechnology 92:551-560 (PDF).
75. Zhang Y-HP*. 2011. Simpler is better: high-yield and potential low-cost biofuels production through cell-free synthetic pathway biotransformation (SyPaB). ACS Catalysis 1: 998-1009 (Invited perspective, PDF).
74. Huang WD, Zhang Y-HP*. 2011. Energy efficiency analysis: biomass-to-wheel efficiency related with biofuels production, fuel distribution, and powertrain systems. PLoS One 6(7): e22113 (Analysis, PDF). A small fraction of the USA biomass resource (i.e., 700 million tons) would be sufficient to replace all gasoline if we increase biomass utilization efficiency by using the new technologies.
73. Zhang XZ, Zhu ZG, Sathitsuksanoh N, Zhang Y-HP*. 2011. One-step production of lactate from cellulose as sole carbon source without any other organic nutrient by recombinant cellulolytic Bacillus subtilis. Metabolic Engineering 13:364-372 (PDF).
72. Zhu ZG, Wang YR, Minteer SD, Zhang Y-HP. 2011. Maltodextrin-powered enzymatic fuel cell through a non-natural enzymatic pathway. Journal of Power Sources 196:7505-7509 (PDF).
71. Myung S, Zhang X-Z, Zhang Y-HP*. 2011. Ultra-stable phosphoglucose isomerase through immobilization of cellulose-binding module-tagged thermophilic enzyme on low-cost high-capacity cellulosic adsorbent. Biotechnology Progress 27:969–975 (PDF).
70. Wang YR, Huang WD, Sathisuksanoh N, Zhu ZG, Zhang Y-HP*. 2011. Biohydrogenation from biomass sugar mediated by cell-free synthetic pathway biotransformation. Chemistry and Biology 18: 372-380 (Featured article, PDF). The lowest cost biohydrogenation and the highest energy-retaining efficiency for jet fuel production.
69. Huang WD, Zhang Y-HP*. 2011. Analysis of biofuels production from sugar based on three criteria: Thermodynamics, bioenergetics, and product separation. Energy & Environmental Science. 4:784-792. (Analysis, PDF). Based on analysis of thermodynamics and bioenergetics, aerobic fermenation cannot be used for competitive biofuels production because a significant fraction of chemical energy in carbohydrate is oxidized by air.
68. Zhang Y-HP*, Mielenz JR. 2011. Renewable hydrogen carrier – carbohydrate: constructing the carbon-neutral carbohydrate economy. Energies 4:254-275 (invited Perspective, PDF).
67. Sathisuksanoh N, Zhu ZG, Zhang Y-HP*. 2011. Cellulose solvent-based pretreatment breaks highly ordered hydrogen bonds in cellulose fibers of switchgrass. Biotechnology and Bioengineering 108:521-529 (PDF).
66. Zhang X-Z, Zhang Y-HP*. 2011. Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis. Microbial Biotechnology 4: 98-105 (Highlighted, PDF).
65. Rollin J, Zhu ZG, Sathisuksanoh N, Zhang Y-HP*. 2011. Increasing substrate accessibility is more important than removing lignin: A comparison of cellulose solvent-based lignocellulose fractionation and soaking in aqueous ammonia. Biotechnology and Bioengineering 108: 22-30 (PDF).
64. Sathisuksanoh N, Zhu Z, Rollin J, Zhang Y-HP*. 2010. Chapter 4. Solvent fractionation of lignocellulosic biomass. Bioalcohol Production (ed. by Keith Waldron). pp122-140 (invited book chapter). Woodheading Publishing and CRC Press, Cambridge, UK.
63. Zhang Y-HP*, Zhu Z, Rollin J, Sathisuksanoh N. 2010. Chapter 20. Advances in cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF). ACS Symposium Series 1033: 365-379 (invited book chapter) in Cellulose solvents: For analysis, shaping and chemical modification, Oxford University Press, UK. ISBN13: 9780841200067.
62. Zhang X-Z*, Zhang Y-HP*. 2010. One-step production of biocommodities from lignocellulosic biomass by recombinant cellulolytic Bacillus subtilis: Opportunities and challenges. Engineering in Life Sciences 10:398-406. (invited review, PDF)
61. Zhang Y-HP*. 2010. Renewable carbohydrates are a potential high density hydrogen carrier. International Journal of Hydrogen Energy 35:10334-10342 (PDF).
60. Liu W, Zhang X-Z, Zhang Z-M, Zhang Y-HP*. 2010. Engineering of Clostridium phytofermentans endoglucanase Cel5A for improved thermostability Applied and Environmental Microbiology 76: 4914-4917 (PDF).
59. Zhang Y-HP*, Sun J-B, Zhong J-J. 2010. Biofuel production by in vitro synthetic enzymatic pathway biotransformation. Current Opinion in Biotechnology 23: 663-669. (invited review, PDF)
58. Myung S, Wang YR, Zhang Y-HP*. 2010. Fructose-1,6-bisphosphatase from a hyper-thermophilic bacterium Thermotoga maritima: Cloning, characterization, metabolite stability, and its implications. Process Biochemistry 45:1882-1887 (PDF).
57. Zhang X-Z, Sathisuksanoh N, Zhang Y-HP*. 2010. Glycoside hydrolase family 9 processive endoglucanase from Clostridium phytofermentans: Heterologous expression, purification, and synergy with family 48 cellobiohydrolase. Bioresource Technology 101:5534-5538 (PDF).
56. Ye X, Zhang Y-HP*. 2010. Thermophilic α-glucan phosphorylase from Clostridium thermocellum: Cloning, characterization, and enhanced thermostability. Journal of Molecular Catalysis B: Enzymatic 65:110–116 (PDF).
55. Zhang Y-HP.* 2010.Production of biocommodities and bioelectricity by cell-free synthetic enzymatic pathway biotransformations: Challenges and opportunities. Biotechnology and Bioengineering 105:663-677 (PDF).
54. Liu W, Bevan DR, Zhang Y-HP*. 2010. The family 1 glycoside hydrolase from Clostridium cellulolyticum H10 is a cellodextrin glucohydrolase. Applied Biochemistry and Biotechnology 161:1-8 (PDF).
53. Sathitsuksanoh N, Zhu ZG, Ho T-J, Bai M-D, Zhang Y-HP*. 2010. Bamboo saccharification through cellulose solvent-based biomass pretreatment followed by enzymatic hydrolysis at ultra-low cellulase loadings. Bioresource Technology 101:4926-4929 (PDF).
52. Zhang XZ, Zhang ZM, Yang D, Zhang Y-HP*. 2010. The non-cellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: Heterologous expression, characterization, and processivity. Applied Microbiology and Biotechnology 86: 525-533 (PDF).
51. Wang Y*, Rollin JA, Zhang Y-HP. 2010. Enhancing allele-specific PRC for specific detection of DNA mutants with nucleotide deletion and insertion. Molecular and Cellular Probes 24:15-19 (PDF).
50. Wang Y, Zhang Y-HP*. 2010. A highly active phosphoglucomutase from Clostridium thermocellum: Cloning, purification, characterization, and enhanced thermostability. Journal of Applied Microbiology 108:39-46 (PDF).
49. Zhang Y-HP*, Hong J, Ye X. 2009. Cellulase Assays. Methods in Molecular Biology 581: 213- 231 (invited book chapter). (ed. by Mielenz JR), Springer-Verlag, New York. ISBN 9781607612131.
48. Zhang Y-HP*, Lynd LR. 2009. New generation biomass conversion: Consolidated bioprocessing. Biomass Recalcitrance: Deconstructing the Plant Cell Wall for Bioenergy (ed. by Himmel, M.E.) Blackwell Publishing.pp 480-493 (invited book chapter). DOI: 10.1002/9781444305418.ch16. ISBN: 9781405163606.
47. Zhang Y-HP*, Feng RL. 2009. Overview of cellulosic ethanol R&D and cellulosic ethanol industry development. 2009 Development Report on Industrial Biotechnology of China. China Science Press. pp54-76 (invited book chapter).
46. Zhang Y-HP*. 2009. Using extremophile enzymes to generate hydrogen for electricity. Microbe 4(12): 560-565 (invited article by the monthly magazine of ASM, PDF).
45. Zhu Z, Sathitsuksanoh N, Zhang Y-HP*. 2009. Direct quantitative determination of adsorbed cellulase on lignocellulosic biomass with its application to study cellulase desorption for potential recycling. Analyst 134:2267-2272 (PDF).
44. Wang Y, Zhang Y-HP*. 2009. Cell-free protein synthesis energized by slowly-metabolized maltodextrin. BMC Biotechnology 9:58 (PDF).
43. Sathitsuksanoh N, Zhu Z, Templeton N, Rollin J, Harvey S, Zhang Y-HP*. 2009. Saccharification of a potential bioenergy crop, Phragmites australis (common reed), by lignocellulose fractionation followed by enzymatic hydrolysis at decreased cellulase loadings. Industrial & Engineering Chemistry Research 48: 6441-6447 (PDF).
42. Liu W, Hong J, Bevan DR, Zhang Y-HP*. 2009. Fast identification of thermostable beta-glucosidase mutants on cellobiose by a novel combinatorial selection/screening approach. Biotechnology and Bioengineering 103:1087-1094. (Highlighted, PDF)
41. Wang Y, Zhang Y-HP*. 2009. Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration. Microbial Cell Factories 8:30 (PDF).
40. Zhang Y-HP*, Sarkanen S, Berson E, Dale BE*. 2009. Pretreatment and biomass recalcitrance: Fundamentals and progress. Applied Biochemistry and Biotechnology 153: 80-83 (PDF).
39. Zhu Z, Sathitsuksanoh N, Vinzant T, Schell DJ, McMillan JD, Zhang Y-HP*. 2009. Comparative study of corn stover pretreated by dilute acid and cellulose solvent-based lignocellulose fractionation: Enzymatic hydrolysis, supramolecular structure, and substrate accessibility. Biotechnology and Bioengineering 103: 715-724 (PDF).
38. Zhang Y-HP*. 2009. A sweet out-of-the-box solution to the hydrogen economy: Is sugar-powered car science fiction? Energy and Environmental Science 2: 272-282 (invited perspective, PDF).
37.Ye X, Wang Y, Hopkins RC, Adams MWW, Evans BR, Mielenz JR, Zhang Y-HP*. 2009. Spontaneous high-yield production of hydrogen from cellulosic materials and water catalyzed by enzyme cocktails. ChemSusChem 2: 149-152 (PDF).
36. Zhang Y-HP*, Wang Y, Ye X. 2008. Biofuels production by cell free synthetic enzymatic technology. Biotechnology: Research, Technology and Applications (ed. by Richter FW) Nova Science Publisher, Hauppauge, NY. pp143-157 (invited book chapter). ISBN 9781604569018.
35. Zhang Y-HP*. 2008. A sweet solution. Public Service Review: Science & Technology 1:150 (invited article).
34. Zhang Y-HP*. 2008. Driving tomorrow by sugars. Public Service Review: Science & Technology 1:126 (invited article).
33. Zhang, Y-HP. 2008. Developing more efficient lignocellulose fractionation technology through in-depth understanding of enzymatic cellulose hydrolysis mechanism. Journal of Biotechnology 136 (Supplement 1):S282-S283 (PDF).
32. Moxley G, Zhu Z, Zhang Y-HP*. 2008. Efficient sugar release by the cellulose solvent based lignocellulose fractionation technology and enzymatic cellulose hydrolysis. Journal of Agricultural and Food Chemistry 56 (17), 7885–7890 (PDF).
31. Hong J, Ye X, Wang Y, Zhang Y-HP*. 2008. Bioseparation of recombinant cellulose binding module-protein by affinity adsorption on an ultra-high-capacity cellulosic adsorbent. Analytica Chimica Acta 621:193-199 (PDF).
30. Hong J, Wang Y, Ye X, Zhang Y-HP*. 2008. Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage. Journal of Chromatography A. 1194(2): 150-154 (PDF).
29. Zhang Y-HP*. 2008. Reviving the carbohydrate economy via multi-product biorefineries. Journal of Industrial Microbiology & Biotechnology 35 (5): 367-375 (invited review, PDF).
28. Lynd LR*, Weimer PJ, Wolfaardt G, Zhang Y-HP. 2007. Chapter 5: Cellulose hydrolysis by Clostridium thermocellum: A microbial perspective. Cellulosome (ed. by Kataeva IA). Nova Science Publisher. Hauppauge, NY. pp 95-117 (invited book chapter). ISBN: 1594549508.
27. Hong J, Ye X, Zhang Y-HP*. 2007. Quantitative determination of cellulose accessibility to cellulase based on adsorption of a non-hydrolytic fusion protein containing CBM and GFP with its applications. Langmuir 23 (25): 12535-12540 (PDF).
26. Moxley G, Zhang Y-HP*. 2007. More accurate determination of acid-labile carbohydrate composition in lignocellulose by modified quantitative saccharification. Energy Fuels 21: 3684-3688 (PDF).
25. Zhang Y-HP*, Evans BR, Mielenz JR, Hopkins RC, Adams MWW. 2007. High-yield hydrogen production from starch and water by synthetic enzymatic pathway. PLoS ONE 2(5): e456 (PDF). This is a seminal paper of in vitro synthetic biology and presents an out-of-the-box solution to the hydrogen economy.
24. Zhang Y-HP*, Ding S-Y, Mielenz JR, Cui J, Elander RT, Laser M, Himmel ME, McMillan JD, Lynd LR. 2007. Fractionating recalcitrant lignocellulose at modest reaction conditions. Biotechnology and Bioengineering 97(2): 214-223. (Accelerated publication, #1 cited paper in 2007, PDF)
23. Zhang Y-HP*, Schell DR, McMillan JD. 2007. Methodological analysis for determination of enzymatic digestibility of cellulosic materials. Biotechnology and Bioengineering 96(1):188-194 (PDF).
22. Lu Y, Zhang Y-HP, Lynd LR*. 2006. Evidence for enzyme-microbe synergy in cellulose utilization by Clostridium thermocellum. Proceedings of the National Academy of Sciences of the USA 103(44): 16165-16169 (PDF).
21. Zhang Y-HP*, Himmel ME, Mielenz JR. 2006. Outlook for cellulase improvement: Screening and selection strategies. Biotechnology Advances 24(5): 452-481. (#1 most cited paper in year 2006, PDF)
20. Zhang Y-HP*, Lynd LR. 2006. A functionally based model for hydrolysis of solid cellulose by fungal cellulase. Biotechnology and Bioengineering 94(5): 888-898 (PDF). This is a milestone mathematical model for enzymatic cellulose hydrolysis.
19. Zhang Y-HP*, Cui XB, Lynd LR, Huang L. 2006. A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: Evidences from supramolecular structures and enzymatic hydrolysis. Biomacromolecules 7(2): 644-648 (PDF).
18. Zhang Y-HP*, Lynd LR. 2006. Biosynthesis of radio-labeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugars. Applied Microbiology and Biotechnology 70(1):123-129 (PDF).
17. Zhang Y-HP, Lynd LR*. 2005. Cellulose utilization by Clostridium thermocellum: Bioenergetics and hydrolysis product assimilation. Proceedings of the National Academy of Sciences of the USA 102: 7321-7325 (PDF). This paper validated biological feasibility of CBP concept.
16. Zhang Y-HP, Lynd LR*. 2005. Determination of the number average degree of polymerization of cellodextrins and cellulose with application to enzymatic cellulose hydrolysis. Biomacromolecules 6(3): 1510-1515 (PDF).
15. Zhang Y-HP, Lynd LR*. 2005. Regulation of cellulase biosynthesis in batch and continuous cultures of Clostridium thermocellum. Journal of Bacteriology 187: 99-106 (PDF).
14. Zhang Y-HP*, Lynd LR*. 2004. Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Non-complexed cellulase systems. Biotechnology and Bioengineering 88: 797-824. (#1 cited paper in 2004, PDF)
13. Zhang Y-HP, Lynd LR*. 2004. Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum. Applied and Environmental Microbiology 70:1563-1569 (PDF).
12. Zhang Y-HP, Lynd LR*. 2003. Cellodextrin production from mixed-acid hydrolysis and chromatographic separation. Analytical Biochemistry 322:225-232.
11. Zhang YH, Lynd LR*. 2003. Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an ELISA-based method with application to Clostridium thermocellum batch Cultures. Analytical Chemistry 75:219-227.
10. Lynd LR*, Zhang YH. 2002. Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: analytical framework and methodological approach. Biotechnology and Bioengineering 77:467-475.
9. Ozkan M, Desai SG., Zhang YH, Stevenson DM, Beane J, Guerinot ML, Lynd LR* 2001. Characterization of thirteen newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria. Journal of Industrial Microbiology Biotechnology 27:275-280.
8. Zhang Y-H, Zhong J-J*. 1997. Hyper-production of ginseng saponin and polysaccharide production by high-density cultivation of Panax notoginseng cells. Enzyme and Microbial Technology 21:58-63.
7. Zhang Y-H, Wang H-Q, Liu S, Yu J-T, Zhong J-J*. 1997. Regulation of apparent viscosity and oxygen transfer coefficient by osmotic pressure in cell suspension of Panax notoginseng. Biotechnology Letters 19: 943-946.
6. Zhong J-J*, Meng X-D, Zhang Y-H, Liu S. 1997. Effective release of ginseng saponin from suspension cells of Panax notoginseng. Biotechnology Techniques 11:241-243.
5. Liu S, Zhong J-J*, Zhang Y-H, Meng X-D. 1997. Effective release of ginseng saponin in cell suspension cultures of Panax notoginseng permeated with dimethyl sulfoxide. Journal of ECUST 23:684-687 (Chinese).
4. Zhang Y-H, Zhong J-J*, Yu J-T. 1997. High-density cultivation of Panax notoginseng cells for production of ginseng saponin and polysaccharide. Journal of ECUST 23:310-314 (Chinese).
3. Zhang Y-H, Zhong J-J*, Yu J-T. 1996. Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: medium sucrose manipulation. Journal of Biotechnology 51: 49-56.
2. Zhang Y-H, Zhong J-J*, Yu J-T. 1996. Effect of nitrogen source on cell growth and ginseng saponin and polysaccharide in suspension cell cultures of Panax notoginseng. Biotechnology Progress 12: 567-571.
1. Zhang Y-H, Zhong J-J*, Yu J-T. 1995. Effects of osmotic pressure on cell growth and ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng. Biotechnology Letters 17: 1347-1350.