Welcome to the Zhang Group Webpage!

The Zhang Lab

Publications

Book Chapters (invited)

B9. Rollin J, Sathisuksanoh N, Zhu Z, Zhang Y-HP*. 2010. Cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF). Wiley Encyclopedia of Energy: Science, Technology and Applications (invited/submitted).

B8. Ye X, Zhang Y-HP*. 2010. High-yield hydrogen generation by self-assembly enzymatic pathways. Wiley Encyclopedia of Energy: Science, Technology and Applications (invited/submitted).

B7. Zhu Z, Sathisuksanoh N, Rollin J, Zhang Y-HP*. 2009. Advances in cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF). ACS Symposium Series (accepted).

B6. Sathisuksanoh N, Zhu Z, Rollin J, Zhang Y-HP*, 2009. Cellulose solvent- and organic solvent-based lignocellulose fractionation (COSLIF). Bioalcohol Production (ed. by Keith Waldron). Woodheading publishing, UK (accepted).

B5. Zhang Y-HP*, Hong J, Ye X. 2009. Cellulase Assays. Methods in Molecular Biology 581: 213- 231(ed. by Mielenz JR), Springer-Verlag, New York.

B4. Zhang Y-HP*, Rui FL. 2009. Overview of cellulosic ethanol R&D and cellulosic ethanol industry development. 2009 Development Report on Industrial Biotechnology of China. China Science Press pp54-76. (English, PDF upon request)

B3. Zhang Y-HP*, Wang Y, Ye X. 2008. Biofuels production by cell free synthetic enzymatic technology. Biotechnology: Research, Technology and Applications (ed. by Richter FW) Nova Science Publishers, Hauppauge, NY. ISBN 9781604569018 (PDF).

B2. Zhang Y-HP*, Lynd LR. 2008. New generation biomass conversion: Consolidated bioprocessing. Biomass Recalcitrance: Deconstructing the Plant Cell Wall for Bioenergy (ed. by Himmel, M.E.) Blackwell Publishing. ISBN: 9781405163606. pp 480-493 (PDF available upon request).

B1. Lynd LR*, Weimer PJ, Wolfaardt G, Zhang Y-HP. 2007. Cellulose hydrolysis by Clostridium thermocellum: A microbial perspective. Cellulosome (ed. by Kataeva, I.A.). Nova Science Publishers. Hauppauge, NY, U.S.A. ISBN: 1594549508. pp 95-117.

Editorial/Commentary/Feature/Letter

4. Zhang Y-HP*. 2009. High-yield generation of hydrogen/electricity by cell-free synthetic pathway biotransformations (SyPaB): Gold mines of extremophilic enzymes and extermophiles. Microbe 5(12), (a monthly ASM magazine) (accepted).
3. Zhang Y-HP*, Rollin JA. 2009. Technical comments on “Greater transportation energy and GHG offsets from bioelectricity than ethanol. Nature Proceedings 2009.3725.1 (PDF).

2. Zhang Y-HP*, 2008. A sweet solution. Public Service Review: Science & Technology 1:150 (PDF).

1. Zhang Y-HP*, 2008. Driving tomorrow by sugars. Public Service Review: Science & Technology 1:126 (PDF).

Peer-reviewed Journal Publications

Year 2010

47. Zhang Y-HP.* 2010. Production of biocommodities and bioelectricity by cell-free synthetic enzymatic pathway biotransformations: Challenges and opportunities. Biotechnology and Bioengineering (revised)
46. Liu W, Bevan DR, Zhang Y-HP*. 2010. The family 1 glycoside hydrolase from Clostridium cellulolyticum H10 is a cellodextrin glucohydrolase. Applied Biochemistry & Biotechnology. (Epub, PDF).
45. Sathitsuksanoh N, Zhu ZG, Ho T-J, Bai M-D, Zhang Y-HP*. 2010. Bamboo saccharification through cellulose solvent-based biomass pretreatment followed by enzymatic hydrolysis at ultra-low cellulase loadings. Bioresource Technology (Epub, PDF).

Year 2009

44. Zhang XZ, Zhang ZM, Sathitsuksanoh N, Yang D, Zhang Y-HP*. 2009. The non-cellulosomal family 48 cellobiohydrolase from Clostridium phytofermentans ISDg: Heterologous expression, characterization, and processivity. Applied Microbiology and Biotechnology (Epub, PDF)

43. Zhu Z, Sathitsuksanoh N, Zhang Y-HP*. 2009. Direct quantitative determination of adsorbed cellulase on lignocellulosic biomass with its application to study cellulase desorption for potential recycling. Analyst (Epub, PDF)

42. Wang Y*, Rollin JA, Zhang Y-HP. 2009. Enhancing allele-specific PRC for specific detection of DNA mutants with nucleotide deletion and insertion. Molecular and Cellular Probes (Epub, PDF).

41. Wang Y, Zhang Y-HP*. 2009. Cell-free protein synthesis energized by slowly-metabolized maltodextrin. BMC Biotechnology 9:59 (PDF).

40. Wang Y, Zhang Y-HP*. 2009. A highly active phosphoglucomutase from Clostridium thermocellum: Cloning, purification, characterization, and enhanced thermostability. Journal of Applied Microbiology (Epub, PDF).

39. Sathitsuksanoh N, Zhu Z, Templeton N, Rollin J, Harvey S, Zhang Y-HP*. 2009. Saccharification of a potential bioenergy crop, Phragmites australis (common reed), by lignocellulose fractionation followed by enzymatic hydrolysis at decreased cellulase loadings. Industrial & Engineering Chemistry Research 48:6441-6447 (PDF).

38. Liu W, Hong J, Bevan DR, Zhang Y-HP*. 2009. Fast identification of thermostable beta-glucosidase mutants on cellobiose by a novel combinatorial selection/screening approach. Biotechnology and Bioengineering 103:1087-1094, (PDF).

37. Wang Y, Zhang Y-H.P*. 2009. Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration. Microbial Cell Factories 8:30 (PDF).

36. Zhang Y-HP*, Sarkanen S, Berson E, Dale BE*. 2009. Sessions 3 and 8: Pretreatment and Biomass Recalcitrance: Fundamentals and Progress. Applied Biotechnology and Biochemistry153: 80-83 (PDF) .

35. Zhu Z, Sathitsuksanoh N, Vinzant T, Schell DJ, McMillan JD, Zhang Y-HP*. 2009. Comparative study of corn stover pretreated by dilute acid and cellulose solvent-based lignocellulose fractionation: Enzymatic hydrolysis, supramolecular structure, and substrate accessibility. Biotechnology and Bioengineering 103: 715-724 ( PDF).

34. Zhang Y-HP*. 2009. Perspective: A sweet out-of-the-box solution for the hydrogen economy: Is sugar-powered car science fiction? Energy Environmental Science 2: 272-282 (PDF). The highest energy efficiency power train system is proposed.

33. Ye X, Wang Y, Hopkins RC, Adams MWW, Evans BR, Mielenz JR, Zhang Y-HP*. 2009. Spontaneous high-yield hydrogen generation from cellulosic materials catalyzed by enzyme cocktails. ChemSusChem 2:149-152. (PDF). Extra chemical energy (hydrogen that we can utilize) is generated from ambient heat for the first time in the history of human beings .

Year 2008

32. Zhang, Y-H P. 2008. Developing more efficient lignocellulose fractionation technology through in-depth understanding of enzymatic cellulose hydrolysis mechanism. Journal of Biotechnology 136 (Supplement 1):S282-S283.
31. Moxley G, Zhu Z, Zhang Y-HP*. 2008. Efficient sugar release by the cellulose solvent-based lignocellulose fractionation technology and enzymatic cellulose hydrolysis. Journal of Agriculture and Food Chemistry 56: 7885–7890 (PDF).
30. Hong J, Ye X, Wang Y, Zhang Y-HP*. 2008. Bioseparation of recombinant cellulose binding module-protein by affinity adsorption on an ultra-high-capacity cellulosic adsorbent. Analytica Chimica Acta 621:193-199 (PDF) .
29. Hong J, Wang Y, Ye X, Zhang Y-HP*. 2008. Simple protein purification through affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage. Journal of Chromatography A. 1194(2): 150-154 (PDF).
28. Zhang Y.-H.P. 2008. Reviving the carbohydrate economy via multi-product biorefineries. Journal of Industrial Microbiology and Biotechnology: 35 (5): 367-375 (PDF).

Year 2007

27. Hong J., Ye X-H., Zhang Y.-H.P. 2007. Quantitative determination of cellulose accessibility to cellulase based on adsorption of a non-hydrolytic fusion protein containing CBM and GFP with its applications. Langmuir 23 (25): 12535-12540 (PDF).
26. Moxley G, Zhang Y.-H.P. 2007. More accurate determination of acid-labile carbohydrate composition in lignocellulose by modified quantitative saccharification. Energy and Fuels 21: 3684-3688 (PDF).

25. Zhang Y.-H.P., Mielenz J.R., Evans B.R., Hopkins R.C., Adams M.W.W. 2007. High-yield hydrogen production from starch and water by synthetic enzymatic pathway. PLoS One 2(5): e456 (PDF). Note: This is a milestone paper for linking the future carbohydrate and hydrogen economy. Also, we proposed a new concept technology -- cell free synthetic enzymatic pathway for complicated biotransformation.

24. Zhang Y.-H.P., Ding S.-Y., Mielenz J.R., Cui J.-.B., Elander R.T., Laser M, Himmel M.E., McMillan, J.D., Lynd, L.R. 2007. Fractionating recalcitrant lignocellulose at modest reaction conditions. Biotechnology and Bioengineering 97(2): 214-223 (PDF). Note: This is the most downloaded paper in year 2007, highlighted as a special Free Download paper selected by ChemWeb and is the most cited paper in year 2007.

23. Zhang Y.-H.P., Schell D.R. McMillan J.D. 2007. Methodological analysis for determination of enzymatic digestibility of cellulosic materials. Biotechnology and Bioengineering 96(1): 188-194 (PDF).

Year 2006

22. Lu Y.P., Zhang Y.-H.P., Lynd L.R. 2006. Evidence for enzyme-microbe synergy in cellulose utilization by Clostridium thermocellum. Proceedings of the National Academy of Sciences of USA 103(44): 16165-16169 (PDF) .
21. Zhang Y.-H.P., Himmel E. M., Mielenz J.R. 2006. Outlook for cellulase improvement: Screening and selection strategies. Biotechnology Advances, 24(5): 452-481 (PDF).
20. Zhang Y.-H.P., Lynd L.R. 2006. A functionally-based model for hydrolysis of solid cellulose by fungal cellulase. Biotechnology and Bioengineering, 94(5): 888-898 (PDF).
19. Zhang Y.-H.P., Cui X.B., Lynd L.R., and Huang L. 2006. A transition from cellulose swelling to cellulose dissolution by o-phosphoric acid: Evidences from supramolecular structures and enzymatic hydrolysis. Biomacromolecules 7(2): 644-648 (PDF).
18. Zhang Y.-H.P., and Lynd L.R. 2006. Biosynthesis of radio-labeled cellodextrins by the Clostridium thermocellum cellobiose and cellodextrin phosphorylases for measurement of intracellular sugars. Applied Microbiology and Biotechnology 70(1):123-129 (PDF).

Year 2005

17. Zhang Y.-H.P., and Lynd L.R. 2005. Cellulose utilization by Clostridium thermocellum: bioenergetics and hydrolysis product assimilation. Proceedings of the National Academy of Sciences of USA 102:7321-7325 (PDF).
16. Zhang Y.-H.P., and Lynd L.R. 2005. Determination of the number average degree of polymerization of cellodextrins and cellulose with application to enzymatic cellulose hydrolysis Biomacromolecules 6: 1510-1515 (PDF).
15. Zhang Y.-H.P., and Lynd L.R. 2005. Regulation of cellulase biosynthesis in batch and continuous cultures of Clostridium thermocellum. Journal of Bacteriology 187:99-106 (PDF).

Year 2004

14. Zhang Y.-H.P., and Lynd L.R. 2004. Toward an aggregated understanding of enzymatic hydrolysis of cellulose: non-complexed cellulase systems. Biotechnology and Bioengineering 88:797-824 (PDF). Note: One of the most downloaded paper (top 25).
13. Zhang Y.-H.P., and Lynd L.R. 2004. Kinetics and relative importance of phosphorolytic and hydrolytic cleavage of cellodextrins and cellobiose in cell extracts of Clostridium thermocellum. Applied and Environmental Microbiology 70:1563-1569 (PDF).

Year 2003

12. Zhang Y.-H.P., and Lynd L.R. 2003. Cellodextrin production from mixed-acid hydrolysis and chromatographic separation. Analytical Biochemistry 332:225-232 (PDF).
11. Zhang Y.-H.P., and Lynd L.R. 2003. Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an ELISA-based method with application to Clostridium thermocellum batch Cultures. Analytical Chemistry 75:219-227 (PDF).

Year 2002

10. Lynd L.R., and Zhang Y.H. 2002. Quantitative determination of cellulase concentration as distinct from cell concentration in studies of microbial cellulose utilization: Analytical framework and methodological approach. Biotechnology and Bioengineering 77:467-475 (PDF).

Year 2001

9. Ozkan M., Desai S.G., Zhang Y.-H., Stevenson D.M., Beane J., Guerinot M.L., and Lynd L.R. 2001. Characterization of thirteen newly isolated strains of anaerobic, cellulolytic, thermophilic bacteria. Journal of Industrial Microbiology Biotechnology 27:275-280 (PDF).

Before Year 2000

8. Zhang Y.-H., and Zhong J.-J. 1997. Hyper-production of ginseng saponin and polysaccharide production by high-density cultivation of Panax notoginseng cells. Enzyme and Microbial Technology 21:58-63 (PDF).
7. Zhang Y.-H., Wang H.-Q., Liu S., Yu J.-T., and Zhong J.-J. 1997. Regulation of apparent viscosity and oxygen transfer coefficient by osmotic pressure in cell suspension of Panax notoginseng. Biotechnology Letters 19: 943-945 (PDF).
6. Zhong J.-J., Meng X.-D., Zhang Y.-H., and Song L. 1997. Effective release of ginseng saponin from suspension cells of Panax notoginseng. Biotechnology Techniques 11:241-243. (PDF)
5. Liu S., Zhong J.-J., Zhang Y.-H., and Meng X.-D. 1997. Effective release of ginseng saponin in cell suspension cultures of Panax notoginseng permeated with dimethyl sulfoxide. Journal of ECUST 23:684-687 (Chinese).
4. Zhang, Y.-H., Zhong, J.-J., and Yu, J.-T. 1997. High-density cultivation of Panax notoginseng cells for production of ginseng saponin and polysaccharide. Journal of ECUST 23:310-314 (Chinese).
3. Zhang, Y.-H., Zhong, J.-J., and Yu, J.-T. 1996. Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: medium sucrose manipulation. Journal of Biotechnology 51: 49-56 (PDF).

2. Zhang Y.-H., Zhong J.-J., and Yu J.-T. 1996. Effect of nitrogen source on cell growth and ginseng saponin and polysaccharide in suspension cell cultures of Panax notoginseng. Biotechnology Progress 12: 567-571 (PDF).
1. Zhang Y.-H., Zhong J.-J., and Yu J.-T. 1995. Effects of osmotic pressure on cell growth and ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng. Biotechnology Letters 17: 1347-1350.

15 Patent Disclosures

Five patent disclosures have been licensed to two biofuels companies.

Selected Presentation Slides
Zhang Y.-H.P. Effectively Fractionating Lignocellulose and Releasing Sugars by Enzymatic Hydrolysis. The 29th Symposium on Biotechnology for Fuels and Chemicals. Nashville, Tennessee, May 2, 2007 (Oral Presentation 5B-05).
Zhang Y.-H.P. Developing zero-pollution cars powered by sugars. Virginia Tech Dean’s Environment Forum. Blacksburg, VA., Feb. 26, 2007. First Place Faculty Award in the Environmental Technologies. (Science Fiction or Reality -- Future Vehicles Powered by Sugar).